pad track sirt1 plasmid Search Results


pad track sirt1  (Addgene inc)
91
Addgene inc pad track sirt1
FIGURE7.A–C,activationofAMPKbyCA-1AMPKexpressionincreasesNAD content and NAD/NADH ratio in macrophages. RAW264.7 macrophages were transfected with CA-1AMPK expression vectors. NAD and NADH nucleotides were measured with a NAD/NADH quantification kit. D and E, activation of AMPK increases <t>SIRT1</t> mRNA expression in macrophages. RAW264.7 macrophages were treated with 2 mM AICAR (D) or transfected with CA-1AMPK overnight (E). F, AICAR increases SIRT1 protein expression in macrophages.PeritonealmacrophagesweretreatedwithAICAR(2mM)atthe indicated time points. SIRT1 mRNA was measured by real time RT-PCR. SIRT1 protein expression was measured by immunoblotting. All data are expressed as mean S.E., n 4; *, p 0.05.
Pad Track Sirt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+plasmid/pAd-Track+SIRT1+(Plasmid+%238440)/10__1074_slash_jbc__m110__123620-70-19-33
Average 91 stars, based on 1 article reviews
pad track sirt1 - by Bioz Stars, 2026-10
91/100 stars
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pad track flag sirt1 h355a  (Addgene inc)
91
Addgene inc pad track flag sirt1 h355a
FIGURE7.A–C,activationofAMPKbyCA-1AMPKexpressionincreasesNAD content and NAD/NADH ratio in macrophages. RAW264.7 macrophages were transfected with CA-1AMPK expression vectors. NAD and NADH nucleotides were measured with a NAD/NADH quantification kit. D and E, activation of AMPK increases <t>SIRT1</t> mRNA expression in macrophages. RAW264.7 macrophages were treated with 2 mM AICAR (D) or transfected with CA-1AMPK overnight (E). F, AICAR increases SIRT1 protein expression in macrophages.PeritonealmacrophagesweretreatedwithAICAR(2mM)atthe indicated time points. SIRT1 mRNA was measured by real time RT-PCR. SIRT1 protein expression was measured by immunoblotting. All data are expressed as mean S.E., n 4; *, p 0.05.
Pad Track Flag Sirt1 H355a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+plasmid/pAd-Track+Flag-SIRT1+H355A+(Plasmid+%238439)/pmc05081691-462-20-23
Average 91 stars, based on 1 article reviews
pad track flag sirt1 h355a - by Bioz Stars, 2026-10
91/100 stars
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sirt1 function hepatocytes  (Addgene inc)
90
Addgene inc sirt1 function hepatocytes
FIGURE7.A–C,activationofAMPKbyCA-1AMPKexpressionincreasesNAD content and NAD/NADH ratio in macrophages. RAW264.7 macrophages were transfected with CA-1AMPK expression vectors. NAD and NADH nucleotides were measured with a NAD/NADH quantification kit. D and E, activation of AMPK increases <t>SIRT1</t> mRNA expression in macrophages. RAW264.7 macrophages were treated with 2 mM AICAR (D) or transfected with CA-1AMPK overnight (E). F, AICAR increases SIRT1 protein expression in macrophages.PeritonealmacrophagesweretreatedwithAICAR(2mM)atthe indicated time points. SIRT1 mRNA was measured by real time RT-PCR. SIRT1 protein expression was measured by immunoblotting. All data are expressed as mean S.E., n 4; *, p 0.05.
Sirt1 Function Hepatocytes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+plasmid/pAd-Track+Flag-SIRT1+(Plasmid+%238438)/pmc03753670-265-2-5
Average 90 stars, based on 1 article reviews
sirt1 function hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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pad track sirt1 h355a  (Addgene inc)
90
Addgene inc pad track sirt1 h355a
FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or <t>SIRT1(H355A).</t> Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.
Pad Track Sirt1 H355a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+plasmid/pAd-Track+SIRT1+H355A+(Plasmid+%238441)/10__1074_slash_jbc__m110__123620-70-21-33
Average 90 stars, based on 1 article reviews
pad track sirt1 h355a - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
sirna sirt1 plasmid  (Addgene inc)
90
Addgene inc sirna sirt1 plasmid
FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or <t>SIRT1(H355A).</t> Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.
Sirna Sirt1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pad+track+sirt1+plasmid/pAd-Track+SIRT1+siRNA+(Plasmid+%238364)/pmc03598082-146-17-16
Average 90 stars, based on 1 article reviews
sirna sirt1 plasmid - by Bioz Stars, 2026-10
90/100 stars
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pad-track flag-sirt1-deltaexon2 (plasmid #105685)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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FIGURE7.A–C,activationofAMPKbyCA-1AMPKexpressionincreasesNAD content and NAD/NADH ratio in macrophages. RAW264.7 macrophages were transfected with CA-1AMPK expression vectors. NAD and NADH nucleotides were measured with a NAD/NADH quantification kit. D and E, activation of AMPK increases SIRT1 mRNA expression in macrophages. RAW264.7 macrophages were treated with 2 mM AICAR (D) or transfected with CA-1AMPK overnight (E). F, AICAR increases SIRT1 protein expression in macrophages.PeritonealmacrophagesweretreatedwithAICAR(2mM)atthe indicated time points. SIRT1 mRNA was measured by real time RT-PCR. SIRT1 protein expression was measured by immunoblotting. All data are expressed as mean S.E., n 4; *, p 0.05.

Journal: Journal of Biological Chemistry

Article Title: Macrophage α1 AMP-activated Protein Kinase (α1AMPK) Antagonizes Fatty Acid-induced Inflammation through SIRT1

doi: 10.1074/jbc.m110.123620

Figure Lengend Snippet: FIGURE7.A–C,activationofAMPKbyCA-1AMPKexpressionincreasesNAD content and NAD/NADH ratio in macrophages. RAW264.7 macrophages were transfected with CA-1AMPK expression vectors. NAD and NADH nucleotides were measured with a NAD/NADH quantification kit. D and E, activation of AMPK increases SIRT1 mRNA expression in macrophages. RAW264.7 macrophages were treated with 2 mM AICAR (D) or transfected with CA-1AMPK overnight (E). F, AICAR increases SIRT1 protein expression in macrophages.PeritonealmacrophagesweretreatedwithAICAR(2mM)atthe indicated time points. SIRT1 mRNA was measured by real time RT-PCR. SIRT1 protein expression was measured by immunoblotting. All data are expressed as mean S.E., n 4; *, p 0.05.

Article Snippet: Plasmid Constructs and Transfection—Construction of constitutively active (CA) and dominant-negative (DN) 1AMPK expression vectors were described previously (20). pCruzSIRT1, pAd-Track SIRT1, pAd-Track-SIRT1 H355A, SIRT2SIRT7, p300, and p65 expression vectors were all purchased from Addgene (Cambridge, MA).

Techniques: Transfection, Expressing, Activation Assay, Quantitative RT-PCR, Western Blot

FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Journal: Journal of Biological Chemistry

Article Title: Macrophage α1 AMP-activated Protein Kinase (α1AMPK) Antagonizes Fatty Acid-induced Inflammation through SIRT1

doi: 10.1074/jbc.m110.123620

Figure Lengend Snippet: FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Article Snippet: Plasmid Constructs and Transfection—Construction of constitutively active (CA) and dominant-negative (DN) 1AMPK expression vectors were described previously (20). pCruzSIRT1, pAd-Track SIRT1, pAd-Track-SIRT1 H355A, SIRT2SIRT7, p300, and p65 expression vectors were all purchased from Addgene (Cambridge, MA).

Techniques: Expressing, Western Blot, Activation Assay, Transfection, Knockdown, Control, Activity Assay

FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Journal: Journal of Biological Chemistry

Article Title: Macrophage α1 AMP-activated Protein Kinase (α1AMPK) Antagonizes Fatty Acid-induced Inflammation through SIRT1

doi: 10.1074/jbc.m110.123620

Figure Lengend Snippet: FIGURE8.A,SIRT1deacetylatesp65inmacrophages.RAW264.7macrophagesweretransfectedwithpcDNA3.1 or expression vectors for p65, p300, SIRT1, or SIRT1(H355A). Cell lysates were used to examine p65 acetylation (lysine 310) by immunoblotting. B and C, activation of AMPK by AICAR (B) or CA-1AMPK (C) deacetylates p65 in macrophages. RAW264.7 macrophages were transfected with expression vectors for p65, p300, and CA-1AMPK, or treated with AICAR (2 mM). D, SIRT1 is required for full capacity of AMPK to deacetylate NF-B. The SIRT1-knockdown or control cells were transfected with expression vectors for p65, p300, and CA-1AMPK. A representative blot was shown in the left panel. The blots were quantitated with a Li-COR Odyssey Infrared Imager system (right panel). *, p 0.05. E and F, SIRT1 is required for full capacity of AMPK to inhibit NF-B transcriptional activity. The control or SIRT1-knockdown macrophages transfected with pNFB-Luc vectors were treated with 2 mM AICAR or co-transfected with CA-1AMPK expression vectors, and then stimulated with stearate (250 M) (E) or LPS (100 ng/ml) (F) for 24 h. All data are expressed as mean S.E., n 4; *, p 0.05.

Article Snippet: Plasmid Constructs and Transfection—Construction of constitutively active (CA) and dominant-negative (DN) 1AMPK expression vectors were described previously (20). pCruzSIRT1, pAd-Track SIRT1, pAd-Track-SIRT1 H355A, SIRT2SIRT7, p300, and p65 expression vectors were all purchased from Addgene (Cambridge, MA).

Techniques: Expressing, Western Blot, Activation Assay, Transfection, Knockdown, Control, Activity Assay